Thakur was suffering from kidney ailments and died at a suburban Andheri hospital, family sources said.
He was 65 and is survived by actor-wife Prita Mathur. Thakur has several acclaimed films like 'Mere Apne', 'Rajanigandha' and 'Ghar' to his credit.
He headed a theatre group called ANK Productions for the last three decades.
Theatre actor-director and Bollywood personality Dinesh Thakur
His oldest and still popular theatre production is 'Jis Lahore Nahi Dekhya'.
Thakur had showcased his play 'Rang Bajrang' at the Prithvi and Tata theatres in April last year.
He was a noted Indian theatre director, actor in theatre, television and Hindi films, where most notably he appeared as one of the leads in 'Rajnigandha' (1974) directed by Basu Chatterjee, which won both Filmfare Best Movie Award and the Filmfare Critics Award for Best Movie.
He appeared in character roles in Hindi films and also was a screenwriter and story writer. He is known for writing the story and screenplay of 'Ghar' (1978), which won him the 1979 Filmfare Best Story Award.
Thakur's last rites will be conducted today at the Oshiwara crematorium around 4.30 pm.