Richard Sears and Nick Gough, currently in Peru, pushed their vehicle the last 43 miles (70km) to break the world record.
The two have travelled at least 23,300 miles (37,500km) in aid of grassroots education projects in Africa, Asia and South America.
According to Sky News, if they are able to travel in their motorised rickshaw through northern Chile they will become the first people to drive a tuk-tuk, which they have named Tommy, around the world.
They set off from London on August 13, 2012 and have crossed 37 countries. Richard Sears, from Guildford, said he hoped the trip would highlight the fact that many children in the developing world still do not have access to education, the report said.
The pair is set to finish their tuk-tuk journey next month in Rio de Janeiro, and they hope the Guinness Book of Records will verify their feat soon after, it added.