Haryana ended the third day at 63-5, chasing 376 in the third round of the tournament after the hosts had declared at 312-5.
Resuming the day at 35 for no loss, opening batsman Unmukt Chand (19), batting with Gambhir, faltered at the start. He was followed by Virender Sehwag (6), who again failed to make an impact.
Later, Gambhir put on an important 80-run third-wicket partnership with Mithun Manhas, who retired hurt on 36. Left hander Vaibhav Rawal (7) also perished quickly.
Ranji round-up: Important scores
In Delhi (Group A): Delhi 201 and 312-5 decl (Gambhir 153, R Bhatia 56 n o; Ashish Hooda 2-38) vs Haryana 138 and 63-5 (Varun Sood 3-3).
In Mohali (Group A): Punjab 172 and 309 (Jiwanjot Singh 51, Uday Kaul 52 n o; Jasprit Bumrah 3-63) vs Gujarat 127 and 33-3.
In Meerut (Group B): Tamil Nadu 297 vs Uttar Pradesh 411-7 (P Chawla batting 114).
In Pune (Group C): Maharashtra 635 (H Khadiwale 262; Amit Yadav 7-155) vs Goa 297-5.
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