Under stormy skies, the world and Olympic champion produced a lightning swim to touch in 1min 48.56sec, nearly half-a-second under his record set in August and five seconds ahead of the pack.
The 21-year-old later added 50m butterfly gold to extend his lead in the overall standings with just two stops left in the eight-leg series after Singapore.
“I just heard the crowd pick up and I knew that I was under world record pace,” said Le Clos.
Photos: 'Dangal' girls Sanya Malhotra, Fatima Sana Shaikh's dinner outing
Photos: Salman Khan, Daisy Shah spotted at the Mumbai airport
Photos: Rakhi Sawant to play Honeypreet in Ram Rahim biopic
Photos: Arvind Kejriwal asks Kamal Haasan to join politics
Photos: TV actress who played goddesses on the small screen