The 12-song concert -- filmed at the Washington Coliseum in 1964 -- was the band's first full US gig, and features performances of "She Loves You" and "Twist And Shout", BBC reported.
It was originally broadcast to two million American cinema-goers in March 1964, a month after it was recorded. The footage then disappeared.
The lost video will now be included in a 92-minute documentary titled "The Beatles: The Lost Concert". Screenvision, who are behind the new movie, say the original master tapes have been restored and remastered.
The film would premiere in New York May 6. It will later be screened in theatres across America.
The first part of the film focuses on the rise of Beatlemania in the US. It contains commentary from Chuck Berry, Mark Ronson, Aerosmith pair Steven Tyler and Joe Perry and Albert Hammond Jr. and Nick Valensi from The Strokes. It is followed by the 12-song set.