The 46-year-old was at Filmistan Studio Sunday with her husband Shriram Nene for the shoot of her show when she saw seven puppies drenched from the previous night's rain.
Madhuri interrupted the shoot, and she along with Nene and the show's director Saahil Chhabria, immediately contacted PETA for help and stayed with the puppies until help arrived, said a statement.
"It’s been raining cats and dogs and many puppies and kittens are now in need of good homes. Madhuri Dixit, Dr Nene and Saahil Chhabria's kind deed will inspire many to come forward to help animals in distress," said PETA India's Sachin Bangera.
The puppies are up for adoption.