Bradley and Phil Mickelson finished off the match by winning four consecutive holes and defeated the former unbeaten pairing of Donald and Garcia.
The US were all square through nine holes against Donald and Garcia in the morning alternate shot matches before they caught fire on the back. On the 245 yard par-three 13th, Bradley hit his tee shot on the green for a sinkable putt. Mickelson got one of the biggest roars of the day from the partisan US gallery when he drained the putt then celebrated with a chest bumped with rising star PGA star Bradley.
The Americans went 3-up on the par-five 609 yard 14th when Garcia missed a six foot putt to the right. That set the stage for Bradley to drain his long put on the par-four 15th to win the match.
Europe ran into trouble on the final hole when Donald put his approach into the bunker.
The Americans fittingly finished it off on the redesigned 15th hole of the Medinah course which US skipper Davis Love set up to try and give his troops the advantage.
Earlier, Tiger Woods and Steve Stricker got off to a rocky start as both hit wayward opening tee shots in their foursome match against Justin Rose and Ian Poulter at the 39th Ryder Cup.