Stuttgart: Sania Mirza and Martina Hingis lost their first competitive match as a women's doubles team as they crashed out of the Porsche Tennis Grand Prix here.
First-time pairing of Petra Martic and Stephanie Vogt pulled of a straight set stunner 6-3, 6-3 on Wednesday.
Martic and Vogt were playing together for the first time and had one Women's Tennis Association (WTA) doubles title between them.
Before Wednesday's defeat, Sania and Martina won back-to-back-to-back titles at Indian Wells, Miami and Charleston, a run which helped the India become the world's No.1 women's doubles player.
Martina was not at her physical peak during the match and was treated for a back injury.
The Indo-Swiss pair are currently No.1 doubles team on the leaderboard for the season-ending WTA championships -the BNP Paribas WTA Finals - to be held in October-November in Singapore, this year.
In other matches, No.2 seeds Caroline Garcia and Katarina Srebotnik beat German pair of Antonia Lottner and Carina Witthoeft, 6-3, 6-3, while No.3 seeds Bethanie Mattek-Sands and Lucie Safarova downed Jocelyn Rae and Anna Smith, 6-2, 6-1.
If Mattek-Sands and Safarova go on to capture the Porsche Tennis Grand Prix title this week, they will pass Sania and Martina the No.1 spot on the leaderboard.