Diane Disney Miller passed away Tuesday after suffering a recent fall, reports a website.
She was the only biological daughter of Disney and his wife, Lillian. Her sister, Sharon Mae, was adopted by the couple in 1936. Mae died 20 years ago.
Disney's daughter was married to former Walt Disney Company CEO Ron W. Miller, who was replaced by Michael Eisner and Frank Wells in 1984.
Paying tribute to Walt's daughter on Tuesday afternoon, current Disney CEO Bob Iger said, "We are deeply saddened by the loss of Diane Disney Miller, and our thoughts are with her family during his difficult time."
"As the beloved daughter of Walt Disney and one of his inspirations for creating Disneyland, she holds a special place in the history of the Walt Disney Company and in the hearts of fans everywhere," he said.
"She will be remembered for her grace and generosity and tireless work to preserve her father’s legacy, and she will be greatly missed by all who knew her."
Photos: Shah Rukh Khan, Rekha at a prestigious awards event
Oscars 2017: Emma Stone, Nicole Kidman, other stars on red carpet
Photos: Varun Dhawan, Alia Bhatt, Amitabh Bachchan walk the ramp
Photos: Priyanka Chopra at Oscars 2017 red carpet
Photos: Ranveer Singh, Ajay Devgn spotted at Mumbai airport