Winfrey earned $165 million between May 2011 and May 2012. Last year also she was on top of the list.
Singer Britney Spears came second with $58 million, after signing up as a judge on "The X Factor", while country singer Taylor Swift landed third place with $57 million, reports contactmusic.com.
Rihanna and Ellen DeGeneres tied with $53 million for fourth spot and Lady Gaga and Jennifer Lopez tied for the fifth with $52 million earnings.
'Rock On 2' trailer launch: Farhan, Shraddha enthrall audience with live gig
Flashback: When bikini babes set a new world record in Australia
Photos: 'Rock On 2' stars Farhan, Shraddha on 'Yaaron Ki Baraat'
Photos: Ajay Devgn launches 'Shivaay' comics at Mumbai Comic Con
Photos: WWE star Sheamus and John Abraham clash in Mumbai